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 Assay

The determination of the protease activity of papain has been a widely disputed subject.

Users are mainly concerned by the enzyme's ability to break down proteins or protein bindings of their own process.
The real effectiveness can only be truly measured on a case by case study on the several products or substrates the enzymes have to act on.

Standard laboratory procedures were developed in order to have reproducible results based on simplified methods and readily available substrates such as milk, gelatine, haemoglobin, and casein.

Later on, new synthetic products such as BAPA (benzol-arginine-p-nitroanilide) and BAEE (benzoyl-arginine-ethylester) became popular substrates because of their invariable reproduction.

Several internationally recognized institutions such as the USP-NF, FCC, the BPC and FIP have been consistently describing and supporting specific testing procedures. In some cases, there exists a correlation between methods.
Results may be expressed in T.U. (tyrosine units), BAPA units, GDU (Gelatine digestion units), HDU (haemoglobin digestion units) and FIP units (International Pharmacopea Federation).

In addition, it is of the greatest importance to mention against which papain standard the measure is taken, generally referred to by standard lot number.
More information on current standard can be obtained at www.usp.org.

Present standard used as reference for ENZYMASE's refined papain is USP lot H against which minimum activity is given at 38.400 USP (±600 T.U.) measured on casein as a substrate. The received activity mostly exceeds 45.000 USP (700 T.U.) certified by an independent laboratory.

More information is available on request at info@enzymase.com.


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