The determination of the protease activity of papain has been
a widely disputed subject.
Users are mainly concerned by the enzyme's ability to break
down proteins or protein bindings of their own process.
The real effectiveness can only be truly measured on a case
by case study on the several products or substrates the enzymes
have to act on.
Standard laboratory procedures were developed in order to
have reproducible results based on simplified methods and
readily available substrates such as milk, gelatine, haemoglobin,
Later on, new synthetic products such as BAPA (benzol-arginine-p-nitroanilide)
and BAEE (benzoyl-arginine-ethylester) became popular substrates
because of their invariable reproduction.
Several internationally recognized institutions such as the
USP-NF, FCC, the BPC and FIP have been consistently describing
and supporting specific testing procedures. In some cases,
there exists a correlation between methods.
Results may be expressed in T.U. (tyrosine units), BAPA units,
GDU (Gelatine digestion units), HDU (haemoglobin digestion
units) and FIP units (International Pharmacopea Federation).
In addition, it is of the greatest importance to mention
against which papain standard the measure is taken, generally
referred to by standard lot number.
More information on current standard can be obtained at www.usp.org.
Present standard used as reference for ENZYMASE's
refined papain is USP lot H against which minimum activity
is given at 38.400 USP (±600 T.U.) measured on casein
as a substrate. The received activity mostly exceeds 45.000
USP (700 T.U.) certified by an independent laboratory.
More information is available on request at firstname.lastname@example.org.